fusionprotein相关论文
Aim: To investigate the regulatory function of Cox7a2 on steroidogenesis and the mechanism involved in TM3 mouse Leydig ......
The construction of recombinant heparinase Ⅱ efficient expression system in E.coli and analysis of r
Heparinase Ⅱ is an important polysaccharide lyase capable of depolymerizing heparin and heparan sulfate,which has a wid......
Evaluation of novel fusion proteins derived from extracellular matrix binding domains of LigB as vac
Leptospira binds to host extracellular matrix (ECM) through surface exposed outer membrane proteins called adhesin in or......
Nanobiomaterials can play a central role in regenerative medicine and tissue engineering by facilitating cellular behavi......
Mitogen-activated protein kinase kinase 6-fusion protein (MAP2K6-FP) potentiates the anti-tumor effe
Objective: To investigate the antitumor effects of a mitogen-activated protein kinase (MAPK) kinase fusion protein,TAT-O......
Hydrophobin HFBI promoting active expression of pediocin PA-1 in Pichia pastoris and extending its a
Pediocin PA-1,originating from Pediococcus acidilactici,is regarded as a broad-spectrum bacteriocin in lactic acid bacte......
Construction of eukaryotic expression plasmid about human retina-derived NT-3 and its expression, se
The human neurotrophin-3(NT-3) DNA was obtained by RT-PCR from retinal tissue.Expression plasmid of NT-3, p EGFP-N1-NT-3......
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The LALF32-51 peptide as component of HPV therapeutic vaccine circumvents the alum-mediated inhibiti
Aluminum-containing adjuvants (alum) continue to be the most widely used adjuvants.It is common knowledge that these adj......
Function of IgD on lymphocyte activation and effect of hIgD-Fc-Ig fusion protein on human PBMC proli
Aim:Investigate the influence of IgD on T/B cell activation and construct hIgDFcIg fusion protein to competitive inhibit......
Protective effect of human CD40-Ig fusion protein in a murine model of acute graft-versus-host disea
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目的制备跨膜型超抗原融合蛋白,研究该蛋白制备的肿瘤疫苗的抗肿瘤作用. 方法用已构建的重组跨膜型超抗原的表达载体pET-28a-TM-SE......
目的 构建表达呼吸道合胞病毒(respiratory syncytial virus,RSV)F蛋白编码基因片段的重组腺病毒.方法 以RSV RNA为模板,利用RT-PC......
提取经植物血凝素诱导培养的我国健康奶牛外周血淋巴细胞总RNA，应用RT—PCR方法扩增出奶牛α-干扰素(BoIFN-α)成熟蛋白基因并将其......
以重组质粒pGAIF为模板,PCR扩增编码细菌素enterocin A的基因片段OAH,克隆到表达载体pMAL-p2x中正确构建重组表达质粒pMA。将pMA转......
目的：比较2种生产基地来源的注射用重组人Ⅱ型肿瘤坏死因子受体-抗体融合蛋白（TNFRIIFc）在中国健康志愿者体内药动学（pharmacokinetics......
目的构建DOC-1R（deleted in oral cancer-1related）的原核表达载体并且纯化其重组蛋白，用于进-步研究DOC-1R蛋白的结构与功能。方法采......
目的分别将人类p100基因，p100的SN基因片段和TD片段定向连入pERFP-CI质粒，使它们可与红色荧光蛋白在HeLa细胞内融合表达，从而为进一步......
针对肿瘤坏死因子（ＴＮＦ）在肿瘤治疗剂量下产生的严重毒副作用及一些肿瘤细胞上的白细胞介素－６（ＩＬ－６）受体明显增高的事实，根据ＴＮＦ结构与功能研究的最新......
以鲑鱼基因组ＤＮＡ为模板，采用ＰＣＲ获得ｓＣＴ基因，并为ＤＮＡ序列分析所证实。以ｐＧＥＸ－３Ｘ为表达载体，利用体外定点突变技术成功地构建了融合蛋白ＧＳＴ－ｓＣＴ的重组表达质粒ｐＧＥＸ－３Ｘ－ｓＣＴ，在大......
目的：将已构建好的新型抗乳腺癌双重靶向性融合蛋白Del1-9-G129R-T3的原核表达载体转化入原核表达宿主，诱导融合蛋白的表达，对之进行......
目的构建EGF-Linker-TCSKDEL融合蛋白表达载体并纯化目的蛋白。方法以天花粉蛋白基因的改造产物TCSKDEL为毒素，人表皮生长因子EGFR......
融合蛋白在临床研究中的应用越来越广泛,尤其是在病毒感染和肿瘤的基因治疗方面,但是其自身也有诸多缺点.鱼精蛋白截短体(tP)因其......
AIM: To study the effect of a novel targeted ribonuclease(TN), the fusion protein of HBVc and human eosinophil-derived n......
Cytochrome C oxidase Ⅲ interacts with hepatitis B virus X protein in vivo by yeast two-hybrid system
AIM: To screen and identify the proteins which interact with hepatitis B virus (HBV) X protein in hepatocytes by yeast t......
Effects of endostatin-vascular endothelial growth inhibitor chimeric recombinant adenoviruses on ant
AIM: To investigate the inhibitory effects of endostatinvascular endothelial growth inhibitor (VEGI151) recombinant aden......
Construction of single chain Fv antibody against transferrin receptor and its protein fusion with al
AIM: To construct fusion protein of a single-chain antibody(scFv) against transferrin receptor (TfR) with alkalinephosph......
Construction, expression and characterization of human interferon α2b-(G4S)n-thymosin α1 fusion prot
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High efficient mammalian expression and secretion of a functional humanized single-chain Fv/human in
AIM: To construct and produce a recombinant bispecific humanized single-chain Fv (sFv) /Interleukin-2 (IL-2)fusion prote......
An efficient fusion protein system for expression ofBacillus anthracis protective antigen as immunog
Objective:To produce high quantities of recombinant protective antigen (rPA) for human vaccine and diagnosis.Methods: Th......
T cell immunoglobulin mucin (TIM) family playsa key role in regulating immune responses.In this study,the interactions o......
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目的获得具有生物学活性的与肿瘤血管导向肽(NGR)融合表达的突变人IL-2蛋白.方法将突变人IL-2与NGR的融合基因重组到表达载体pET-2......
目的 以精氨酸-甘氨酸-天冬氨酸(RGD)多肽重复序列作为截短组织因子(tTF)的载体,表达(RGD)_3-tTF融合蛋白,研究(RGD)_3-tTF融合蛋......
目的研究人B7.2分子中IgV和IgC 结构域何者含有与受体结合的位点；对由原核系统表达的B7.2蛋白体外共刺激活性作出评价。 方法 用PC......
目的 应用RNA干扰技术抑制Kasumi-1细胞AML1-ETO融合基因的表达,研究随后出现的细胞增殖和细胞周期变化.方法 体外化学合成针对AML......
目的 探讨甲异靛对K562细胞bcr-abl信号通路的影响以及甲异靛诱导K562细胞凋亡的机制.方法 应用流式细胞术检测甲异靛诱导K562细胞......
Objective: To express the soluble recombinant hemangiopoietin protein in E. coli BL21(DE3). Methods: Using human fetal l......
,Efficient growth inhibition of ErbB2-overexpressing tumor cells by anti-ErbB2 ScFv-Fc-IL-2 fusion p
Aim: To investigate the antitumor activities of an anti-ErbB2 scFv-Fc-interleukin 2 (IL-2) fusion protein (HFI) in vitro......
目的获得人 Fas L蛋白 ,并对其功能进行初步研究。方法用 DNA重组法构建了 Fas L c DNA和谷胱甘肽转硫酶融合原核表达质粒 p GEX- ......
对重组乙型肝炎(乙肝)病毒前S1抗原(1-42)及核心抗原(1-144)表达的融合蛋白CS1进行了免疫原性的研究分析,以便为探索HBV治疗性疫苗......
TNF与多种疾病密切相关。为了获得大量具有生物学活性的可溶性TNF受体用以拮抗TNF的毒性作用，在原核表达系统中表达了TNFR（P55）的胞外......
为获得高质量及充足的Ｆａｓ蛋白，采用ＰＣＲ技术调整Ｆａｓ基因的开放阅读框架，使之与生物素化蛋白基因阅读框架一致；缺失了ＦａｓｃＤＮＡ基因的起始密码子并增加一个......
目的 构建小鼠白细胞介素－１２的融合基因，并进行初号表达，方法 通过重叠序列引物的设计与多聚酶链反应（ＰＣＲ）技术，将小鼠ＩＬ－１２的４０ｋＰａ多肽链基因与３５ｋＤａ多肽链......
探索ＧＰＩ－锚固蛋白的体外表达，获得具有生物学活性的可溶性游离ＣＤ５９分子。方法通过ＰＣＲ选择性扩增编码成熟ＣＤ５９氨基酸序列的基因片段，并将之克隆入ＰｉｎＰｏｉｎｔＸａ－３质粒......
目的：用ｐＥＴ２１原核表达系统表达癌胚抗原单链抗体－链亲和素融合蛋白。方法：融合表达载体转化大肠杆菌菌株ＨＭＳ１７４（ＤＥ３），用１ｍｍｏｌ／Ｌ的ＩＰＴＧ诱导表达，表达产物行亲和印迹及......
ＩＬ－２－ＰＥ６６４Ｇｌｕ是由白介素２同改构的绿脓杆菌外毒素通过基因融合构成的嵌合蛋白。我们研究了它对ＣｏｎＡ诱导的小鼠脾母细胞和ＰＨＡ激活的人外周血淋巴细胞的细......
